Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations

Sonya A. MacParland; Jeff C. Liu; Xue‐Zhong Ma; Brendan T. Innes; Agata Bartczak; Blair K. Gage; Justin Manuel; Nicholas Khuu; Juan Echeverri; Ivan Linares; Rahul Gupta; Michael Cheng; Lewis Liu; Damra Camat; Sai Chung; Rebecca K. Seliga; Zigong Shao; Elizabeth Lee; Shinichiro Ogawa; Mina Ogawa; Michael D. Wilson; Jason E. Fish; Markus Selzner; Anand Ghanekar; David Grant; Paul D. Greig; Gonzalo Sapisochín; Nazia Selzner; Neil Winegarden; Oyedele Adeyi; Gordon Keller; Gary D. Bader; Ian D. McGilvray · 2018 · Nature Communications

Paper

Abstract

The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal cells obtained from the fractionation of fresh hepatic tissue from five human livers. Using gene expression patterns, flow cytometry, and immunohistochemical examinations, we identify 20 discrete cell populations of hepatocytes, endothelial cells, cholangiocytes, hepatic stellate cells, B cells, conventional and non-conventional T cells, NK-like cells, and distinct intrahepatic monocyte/macrophage populations. Together, our study presents a comprehensive view of the human liver at single-cell resolution that outlines the characteristics of resident cells in the liver, and in particular provides a map of the human hepatic immune microenvironment.

Analysis

Paper intelligence

Summary

This study utilized single-cell RNA sequencing to map the cellular landscape of the human liver, identifying 20 distinct cell populations and characterizing the hepatic immune microenvironment.

Key findings

A map of the cellular landscape of the human liver was generated using single-cell RNA sequencing.
Transcriptional profiles of 8444 parenchymal and non-parenchymal cells were obtained.
Twenty discrete cell populations were identified, including hepatocytes, endothelial cells, cholangiocytes, hepatic stellate cells, and various immune cells.
Distinct intrahepatic monocyte/macrophage populations were characterized.

Methods

Single-cell RNA sequencing of human hepatic tissue.
Gene expression pattern analysis.
Flow cytometry.
Immunohistochemical examinations.

Limitations

The study analyzed tissue from five human livers, which may not represent the full spectrum of human liver diversity.
The study focused on transcriptional profiles, which may not fully capture functional differences between cells.

Applications

Understanding liver development and function.
Investigating liver diseases and immune responses.
Developing targeted therapies for liver conditions.

Confidence90%

Discovery

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Source record

Publication metadata

Authors: Sonya A. MacParland; Jeff C. Liu; Xue‐Zhong Ma; Brendan T. Innes; Agata Bartczak; Blair K. Gage; Justin Manuel; Nicholas Khuu; Juan Echeverri; Ivan Linares; Rahul Gupta; Michael Cheng; Lewis Liu; Damra Camat; Sai Chung; Rebecca K. Seliga; Zigong Shao; Elizabeth Lee; Shinichiro Ogawa; Mina Ogawa; Michael D. Wilson; Jason E. Fish; Markus Selzner; Anand Ghanekar; David Grant; Paul D. Greig; Gonzalo Sapisochín; Nazia Selzner; Neil Winegarden; Oyedele Adeyi; Gordon Keller; Gary D. Bader; Ian D. McGilvray
Published: Oct 16, 2018
Journal or venue: Nature Communications
DOI: 10.1038/s41467-018-06318-7
Paper type: journal article
Source: Openalex

DOI OpenAlex